SRY monoclonal antibody, clone SRY.G12
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More Files
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Immunoprecipitation
SRY protein was immunoprecipitated from 1 mg total mouse XY testes (Lane 1) or mesonephros (Lane 2) tissue with a rabbit anti SRY antibody, followed by western blot analysis using SRY monoclonal antibody, clone SRY.G12 (Cat # MAB8814).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant SRY.
Immunogen
Recombinant fusion GST protein corresponding to human SRY.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A purification
Concentration
1 ug/uL
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
SRY protein was immunoprecipitated from 1 mg total mouse XY testes (Lane 1) or mesonephros (Lane 2) tissue with a rabbit anti SRY antibody, followed by western blot analysis using SRY monoclonal antibody, clone SRY.G12 (Cat # MAB8814).Enzyme-linked Immunoabsorbent Assay
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Gene Info — SRY
Entrez GeneID
6736Gene Name
SRY
Gene Alias
TDF, TDY
Gene Description
sex determining region Y
Omim ID
480000Gene Ontology
HyperlinkGene Summary
This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000034324|essential protein for sex determination in human males|sex determining region protein|sex-determining region on Y|testis-determining factor
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Interactome
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Disease
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Publication Reference
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Phospho-ΔNp63α/microRNA feedback regulation in squamous carcinoma cells upon cisplatin exposure.
Huang Y, Kesselman D, Kizub D, Guerrero-Preston R, Ratovitski EA.
Cell Cycle 2013 Feb; 12(4):684.
Application：WB, Human, SCC-11 cells.
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Phospho-ΔNp63α/microRNA feedback regulation in squamous carcinoma cells upon cisplatin exposure.
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