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Last updated: 2017/9/24
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SUMO2 monoclonal antibody, clone AT10F1

  • Catalog # : MAB8754
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant SUMO2.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 1-93 of human SUMO2.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA (1:250)
    Western Blot (1:250-1:500)
    Immunofluorescence (1:250-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of cell lysates of HeLa (35 ug) and recombinant proteins (10 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
    Lane 1: HeLa
    Lane 2: Sumo 2
    Lane 3: Sumo 3
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 500) with Texas Red (Red).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunocytochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 6613
  • Gene Name:
  • SUMO2
  • Gene Alias:
  • HSMT3,MGC117191,SMT3B,SMT3H2
  • Gene Description:
  • SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
  • Gene Summary:
  • This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
  • Other Designations:
  • SMT3 suppressor of mif two 3 homolog 2,sentrin 2,small ubiquitin-like modifier 2, isoform a
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