SUMO2 monoclonal antibody, clone AT10F1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: K562 cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 500) with Texas Red (Red).
Flow Cytometry
Flow cytometry analysis of jurkat cell line, staining at 2-5 ug for 1x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant SUMO2.
Immunogen
Recombinant protein corresponding to amino acids 1-93 of human SUMO2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: Jurkat cell lysate, Lane 3: K562 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 500) with Texas Red (Red).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of jurkat cell line, staining at 2-5 ug for 1x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — SUMO2
Entrez GeneID
6613Protein Accession#
NP_008868Gene Name
SUMO2
Gene Alias
HSMT3, MGC117191, SMT3B, SMT3H2
Gene Description
SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
Omim ID
603042Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
SMT3 suppressor of mif two 3 homolog 2|sentrin 2|small ubiquitin-like modifier 2, isoform a
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