Western blot analysis of cell lysates of HeLa (35 ug) and recombinant proteins (10 ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: HeLa Lane 2: Sumo 2 Lane 3: Sumo 3
Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and SUMO2 monoclonal antibody, clone AT10F1 (Cat # MAB8754) (1 : 500) with Texas Red (Red).
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
SMT3 suppressor of mif two 3 homolog 2,sentrin 2,small ubiquitin-like modifier 2, isoform a