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Last updated: 2021/1/17
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BAK1 monoclonal antibody, clone AT38E2 

  • Catalog # : MAB8753
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant BAK1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 29-187 of human BAK1.
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2a, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: 293T cell lysate, Lane 2: HeLa cell lysate, Lane 3: A431 cell lysate, Lane 4: A549 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: MCF7 cell lysate, Lane 7: PC3 cell lysate.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and BAK1 monoclonal antibody, clone AT38E2 (Cat # MAB8753) (1 : 500) with Texas Red (Red).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
  • Application Image
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 578
  • Gene Name:
  • BAK1
  • Gene Alias:
  • BAK,BAK-LIKE,BCL2L7,CDN1,MGC117255,MGC3887
  • Gene Description:
  • BCL2-antagonist/killer 1
  • Gene Summary:
  • The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq
  • Other Designations:
  • BCL2-like 7 protein,Bcl-2 homologous antagonist/killer,OTTHUMP00000016211,OTTHUMP00000039620,apoptosis regulator BAK,pro-apoptotic protein BAK
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