Western blot analysis of Lane 1: 293T cell lysate, Lane 2: HeLa cell lysate, Lane 3: A431 cell lysate, Lane 4: A549 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: MCF7 cell lysate, Lane 7: PC3 cell lysate.
Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and BAK1 monoclonal antibody, clone AT38E2 (Cat # MAB8753) (1 : 500) with Texas Red (Red).
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq
Other Designations:
BCL2-like 7 protein,Bcl-2 homologous antagonist/killer,OTTHUMP00000016211,OTTHUMP00000039620,apoptosis regulator BAK,pro-apoptotic protein BAK