GTF2B monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of cell lysate using GTF2B monoclonal antibody (Cat # MAB8722) at 1 : 1000 dilution. Lane 1 : Jurkat whole cell lysate 40 ug, Lane2 : MCF-7 whole cell lysate 40 ug, Predicted band size : 35 KDa, Observed band size : 37 KDa.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GTF2B.
Immunogen
Recombinant protein corresponding to full length human GTF2B.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to GTF2B.
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of cell lysate using GTF2B monoclonal antibody (Cat # MAB8722) at 1 : 1000 dilution. Lane 1 : Jurkat whole cell lysate 40 ug, Lane2 : MCF-7 whole cell lysate 40 ug, Predicted band size : 35 KDa, Observed band size : 37 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GTF2B
Entrez GeneID
2959GeneBank Accession#
NM_001514Gene Name
GTF2B
Gene Alias
TF2B, TFIIB
Gene Description
general transcription factor IIB
Omim ID
189963Gene Ontology
HyperlinkGene Summary
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq
Other Designations
OTTHUMP00000012347|OTTHUMP00000058838|RNA polymerase II transcription factor IIB
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Interactome
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Pathway
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