PPP1CA monoclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of PPP1CA monoclonal antibody (Cat # MAB8715) at 1 : 1000 dilution. Lane1 : MCF-7 whole cell lysate 30 ug/lane. Lane 2 : K-562 whole cell lysate 30 ug/lane. Lane 3 : HeLa whole cell lysate 30 ug/lane. Lane 4 : HepG2 whole cell lysate 30 ug/lane. Lane 5 : Jurkat whole cell lysate 30 ug/lane. Predicted band size : 38 KDa. Observed band size : 38 KDa.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant PPP1CA.
Immunogen
Recombinant protein corresponding to full length human PPP1CA.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to PPP1CA.
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:100-1:2000)
ELISA (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PPP1CA monoclonal antibody (Cat # MAB8715) at 1 : 1000 dilution. Lane1 : MCF-7 whole cell lysate 30 ug/lane. Lane 2 : K-562 whole cell lysate 30 ug/lane. Lane 3 : HeLa whole cell lysate 30 ug/lane. Lane 4 : HepG2 whole cell lysate 30 ug/lane. Lane 5 : Jurkat whole cell lysate 30 ug/lane. Predicted band size : 38 KDa. Observed band size : 38 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PPP1CA
Entrez GeneID
5499GeneBank Accession#
NM_001008709Gene Name
PPP1CA
Gene Alias
MGC15877, MGC1674, PP-1A, PPP1A
Gene Description
protein phosphatase 1, catalytic subunit, alpha isoform
Omim ID
176875Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
protein phosphatase 1, catalytic subunit, alpha|serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
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