KDM1A monoclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of KDM1A monoclonal antibody (Cat # MAB8709) at 1 : 1000 dilution. Lane 1 : MCF-7 whole cell lysate 40 ug/Lane. Lane 2 : NIH/3T3 whole cell lysate 40 ug/Lane. Lane 3 : 293T whole cell lysate 40 ug/Lane. Predicted band size : 95 KDa. Observed band size : 50 KDa.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant KDM1A.
Immunogen
Recombinant protein corresponding to internal residues of human KDM1A.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to KDM1A.
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:100-1:2000)
ELISA (1:5000-1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of KDM1A monoclonal antibody (Cat # MAB8709) at 1 : 1000 dilution. Lane 1 : MCF-7 whole cell lysate 40 ug/Lane. Lane 2 : NIH/3T3 whole cell lysate 40 ug/Lane. Lane 3 : 293T whole cell lysate 40 ug/Lane. Predicted band size : 95 KDa. Observed band size : 50 KDa.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AOF2
Entrez GeneID
23028GeneBank Accession#
NM_001009999Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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