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Last updated: 2021/1/24
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GAPDH monoclonal antibody, clone AT8G4 

  • Catalog # : MAB8701
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant GAPDH.
  • Immunogen:
  • Recombinant protein corresponding to full length human GAPDH.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Concentration:
  • 1 mg/mL
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot analysis of Lane 1: Anti-human GAPDH antibody (1:1000), Lane 2: Anti-human GAPDH antibody (1:2000), Lane 3: Anti-human GAPDH antibody (1:4000), Lane 4: Anti-human GAPDH antibody (1:6000), Lane 5: Anti-human GAPDH antibody (1:8000), Lane 6: Anti-human GAPDH antibody (1:10000).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and GAPDH monoclonal antibody, clone AT8G4 (Cat # MAB8701) (1 : 500) with Texas Red (Red).
  • Enzyme-linked Immunoabsorbent Assay
  • Application Image
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 2597
  • Gene Name:
  • GAPDH
  • Gene Alias:
  • G3PD,GAPD,MGC88685
  • Gene Description:
  • glyceraldehyde-3-phosphate dehydrogenase
  • Gene Summary:
  • The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174431,OTTHUMP00000174432,aging-associated gene 9 protein,glyceraldehyde 3-phosphate dehydrogenase
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