Western blot analysis from cell lysate of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with LDHA monoclonal antibody, clone AT1A4 (Cat # MAB8700) (1 : 8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and LDHA monoclonal antibody, clone AT1A4 (Cat # MAB8700) (1 : 500) with Texas Red (Red).
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq