RHO monoclonal antibody, clone 4D2
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Specification
Product Description
Mouse monoclonal antibody raised against full length native RHO.
Immunogen
Native purified bovine RHO.
Host
Mouse
Reactivity
Bovine, Mammals
Specificity
This antibody is specific to N-terminus of RHO.
Form
Liquid
Recommend Usage
Western Blot (1:1000)
Immunoprecipitation(1-2 ug/100-500 ug protein)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — RHO
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Publication Reference
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Covalent bond between ligand and receptor required for efficient activation in rhodopsin.
Matsuyama T, Yamashita T, Imai H, Shichida Y.
The Journal of Biological Chemistry 2009 Dec; 285(11):8114.
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Helix formation in arrestin accompanies recognition of photoactivated rhodopsin.
Feuerstein SE, Pulvermuller A, Hartmann R, Granzin J, Stoldt M, Henklein P, Ernst OP, Heck M, Willbold D, Koenig BW.
Biochemistry 2009 Nov; 48(45):10733.
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Retinitis pigmentosa associated with rhodopsin mutations: Correlation between phenotypic variability and molecular effects.
Iannaccone A, Man D, Waseem N, Jennings BJ, Ganapathiraju M, Gallaher K, Reese E, Bhattacharya SS, Klein-Seetharaman J.
Vision Research 2006 Dec; 46(27):4556.
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Examining rhodopsin folding and assembly through expression of polypeptide fragments.
Ridge KD, Lee SS, Abdulaev NG.
The Journal of Biological Chemistry 1996 Mar; 271(13):7860.
Application:WB-Re, WB-Tr, Monkey, COS-1 cells, Recombinant proteins.
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Peripherin. A rim-specific membrane protein of rod outer segment discs.
Molday RS, Hicks D, Molday L.
Investigative Ophthalmology & Visual Science 1987 Jan; 28(1):50.
Application:IS, Bovine, Retinas.
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Covalent bond between ligand and receptor required for efficient activation in rhodopsin.
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