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Last updated: 2016/12/4
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EGLN1 monoclonal antibody, clone AT1E9

  • Catalog # : MAB8483
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant EGLN1.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 271-426 of human EGLN1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Recommended dilution starting at 1:500.
    Western blot (1:500-1000)
    Immunofluorescence (1:500-1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C or lower..
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with EGLN1 monoclonal antibody, clone AT1E9 (Cat # MAB8483) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunofluorescence
  • Immunofluorescence
  • HeLa cells were stained with EGLN1 monoclonal antibody, clone AT1E9 (Cat # MAB8483) (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • EGLN1
  • Gene Alias:
  • C1orf12,DKFZp761F179,ECYT3,HIFPH2,HPH2,PHD2,SM-20,SM20,ZMYND6
  • Gene Description:
  • egl nine homolog 1 (C. elegans)
  • Other Designations:
  • HIF prolyl hydroxylase 2,egl nine homolog 1,egl nine-like protein 1
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