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CLEC4E monoclonal antibody, clone AT16E3

  • Catalog # : MAB8479
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant CLEC4E.
  • Immunogen:
  • Recombinant protein corresponding to amino acids 41-219 of human CLEC4E.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA (1:250)
    Western Blot (1:250-1:500)
    Immunofluorescence (1:250-1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (0.02% sodium azide, 10% glycerol)
  • Storage Instruction:
  • Store at 4°C short term. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry paraffin embedded sections of human colorectal cancer tissue were incubated with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of human HeLa cells stained with monoclonal CLEC4E monoclonal antibody, clone AT16E3 (Cat # MAB8479) (1 : 500) with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • CLEC4E
  • Gene Alias:
  • CLECSF9,MINCLE
  • Gene Description:
  • C-type lectin domain family 4, member E
  • Gene Summary:
  • This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type II transmembrane protein is a downstream target of CCAAT/enhancer binding protein (C/EBP), beta (CEBPB) and may play a role in inflammation. Alternative splice variants have been described but their full-length sequence has not been determined. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq
  • Other Designations:
  • C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 9,macrophage-inducible C-type lectin
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