This antibody has been directly tested for reactivity in human, mouse and rat. This antibody is specific to the ~ 28KDa Cdk5 protein in Western blots of rat striatal lysate. This antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured rat neurons and for immunohistochemistry on mouse brain tissue.
Protein G purification
Western Blot (1:1000) Immunohistochemistry (1:500) The optimal working dilution should be determined by the end user.
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.