CDK5 monoclonal antibody, clone 1H3
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat caudate lysate showing specific immunolabeling of the ~ 28k CDK5 protein. Using CDK5 monoclonal antibody, clone 1H3 (Cat # MAB8433).
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Specification
Product Description
Mouse monoclonal antibody raised against CDK5.
Immunogen
Purified CDK5.
Host
Mouse
Theoretical MW (kDa)
28
Reactivity
Human, Mouse, Rat
Specificity
This antibody has been directly tested for reactivity in human, mouse and rat. This antibody is specific to the ~ 28KDa Cdk5 protein in Western blots of rat striatal lysate. This antibody shows no cross reactivity with the Cdk5 cofactor p35 or its degradation product p25. This monoclonal also works well for immunocytochemistry using primary cultured rat neurons and for immunohistochemistry on mouse brain tissue.
Form
Liquid
Purification
Protein G purification
Isotype
IgG
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat caudate lysate showing specific immunolabeling of the ~ 28k CDK5 protein. Using CDK5 monoclonal antibody, clone 1H3 (Cat # MAB8433).Immunohistochemistry
Immunocytochemistry
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Publication Reference
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Cdk5 is essential for adult hippocampal neurogenesis.
Lagace DC, Benavides DR, Kansy JW, Mapelli M, Greengard P, Bibb JA, Eisch AJ.
PNAS 2008 Nov; 105(47):18567.
Application：IF, IHC, WB, Mouse, Neurons in the hippocampus of mouse.
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Identification of tyrosine hydroxylase as a physiological substrate for Cdk5.
Kansy JW, Daubner SC, Nishi A, Sotogaku N, Lloyd MD, Nguyen C, Lu L, Haycock JW, Hope BT, Fitzpatrick PF, Bibb JA.
Journal of Neurochemistry 2004 Oct; 91(2):374.
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Cdk5 and the mystery of synaptic vesicle endocytosis.
Nguyen C, Bibb JA.
International Journal of Cell Biology 2003 Nov; 163(4):697.
Application：WB, Human, Mouse, Mammalian cells.
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Cdk5 is essential for adult hippocampal neurogenesis.
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