Zp3r monoclonal antibody, clone 5F12
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Specification
Product Description
Mouse monoclonal antibody raised against Zp3r.
Immunogen
A mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient.
Host
Mouse
Reactivity
Mouse
Specificity
These antibodies specifically recognize mouse sperm protein sp56. They will react with denatured, nonreduced sp56 but not with denatured, reduced sp56. These antibodies do not cross-react with human sperm.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — Zp3r
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Publication Reference
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Tissue- and species-specific expression of sp56, a mouse sperm fertilization protein.
Bookbinder LH, Cheng A, Bleil JD.
Science 1995 Jul; 269(5220):86.
Application：IF, IHC-P, WB-Ce, WB-Ti, Mouse, Eggs, Epididymus, Embryos, Testes.
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Sperm-egg recognition in the mouse: characterization of sp56, a sperm protein having specific affinity for ZP3.
Cheng A, Le T, Palacios M, Bookbinder LH, Wassarman PM, Suzuki F, Bleil JD.
The Journal of Cell Biology 1994 May; 125(4):867.
Application：ELISA, WB-Ce, Mouse, Mouse sperms.
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Tissue- and species-specific expression of sp56, a mouse sperm fertilization protein.
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