PCSK9 (prodomain) monoclonal antibody, clone KS-2C8
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More Files
- More Functions
Western Blot
Western blot analysis of PCSK9 (prodomain) monoclonal antibody, clone KS-2C8 (Cat # MAB8228).
Lane 1: purified PCSK9-His
Lane 2 : PCSK9-His expressing 293 cell
Lane 3 : HepG2 culture supernatant
Lane 4 : HepG2 cell
Lane 5 : A-431 cell
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant PCSK9.
Immunogen
Recombinant His fusion protein corresponding to full length human PCSK9.
Host
Mouse
Reactivity
Human
Specificity
This antibody detects endogenous mature form of PCSK9 prodomain, does not react with pro-form of PCSK9.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (0.5-1 ug/mL)
Immunoprecipitation (1-2 ug/sample)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM phosphate buffer, 150 mM NaCl, pH 7.5 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of PCSK9 (prodomain) monoclonal antibody, clone KS-2C8 (Cat # MAB8228).
Lane 1: purified PCSK9-His
Lane 2 : PCSK9-His expressing 293 cell
Lane 3 : HepG2 culture supernatant
Lane 4 : HepG2 cell
Lane 5 : A-431 cellImmunoprecipitation
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Gene Info — PCSK9
Entrez GeneID
255738Gene Name
PCSK9
Gene Alias
FH3, HCHOLA3, LDLCQ1, NARC-1, NARC1
Gene Description
proprotein convertase subtilisin/kexin type 9
Gene Ontology
HyperlinkGene Summary
This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. The encoded protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. This protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a third form of autosomal dominant familial hypercholesterolemia (HCHOLA3). [provided by RefSeq
Other Designations
OTTHUMP00000009833|convertase subtilisin/kexin type 9 preproprotein|neural apoptosis regulated convertase 1
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Interactome
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Disease
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Publication Reference
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Binding of proprotein convertase subtilisin/kexin type 9 to epidermal growth factor-like repeat A of low density lipoprotein receptor decreases receptor recycling and increases degradation.
Zhang DW, Lagace TA, Garuti R, Zhao Z, McDonald M, Horton JD, Cohen JC, Hobbs HH.
The Journal of Biological Chemistry 2007 Jun; 282(25):18602.
Application：WB-Tr, Human, Monkey, HEK 293, Simian COS-M cells.
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Secreted PCSK9 decreases the number of LDL receptors in hepatocytes and in livers of parabiotic mice.
Lagace TA, Curtis DE, Garuti R, McNutt MC, Park SW, Prather HB, Anderson NN, Ho YK, Hammer RE, Horton JD.
The Journal of Clinical Investigation 2006 Nov; 116(11):2995.
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Effect of mutations in the PCSK9 gene on the cell surface LDL receptors.
Cameron J, Holla OL, Ranheim T, Kulseth MA, Berge KE, Leren TP.
Human Molecular Genetics 2006 May; 15(9):1551.
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Binding of proprotein convertase subtilisin/kexin type 9 to epidermal growth factor-like repeat A of low density lipoprotein receptor decreases receptor recycling and increases degradation.
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