SERPINB5 monoclonal antibody, clone AT2N6
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant SERPINB5.
Immunogen
Recombinant protein corresponding to full length human SERPINB5.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis from cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with SERPINB5 monoclonal antibody, clone AT2N6 (Cat # MAB8227) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with PI (Red) and SERPINB5 monoclonal antibody, clone AT2N6 (Cat # MAB8227) (1 : 500) with Alexa 488 (Green).Enzyme-linked Immunoabsorbent Assay
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Gene Info — SERPINB5
Entrez GeneID
5268Protein Accession#
NP_002630Gene Name
SERPINB5
Gene Alias
PI5, maspin
Gene Description
serpin peptidase inhibitor, clade B (ovalbumin), member 5
Omim ID
154790Gene Ontology
HyperlinkGene Summary
clade B (ovalbumin)
Other Designations
protease inhibitor 5 (maspin)|serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
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Interactome
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Pathway
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Publication Reference
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Maspin: the new frontier.
Khalkhali-Ellis Z.
Clinical Cancer Research 2006 Dec; 12(24):7279.
Application:IHC, WB, Human, Human mammalian cells, Tumors.
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Tumor suppressive maspin and epithelial homeostasis.
Lockett J, Yin S, Li X, Meng Y, Sheng S.
Biochemistry 2006 Mar; 97(4):651.
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Maspin: the new frontier.
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