DYKDDDDK tag monoclonal antibody, clone G191
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of extract from 293 cells transfected with (Lane 1) or without (Lane 2) Flag-tagged Bad using DYKDDDDK tag monoclonal antibody, clone G191 (Cat # MAB8183).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptides of DYKDDDDK tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to DYKDDDDK tag.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes over-expressed proteins containing Flag epitope tag (D-tag) fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Form
Liquid
Recommend Usage
Western Blot (1:1000-1:4000)
Immunoprecipitation (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of extract from 293 cells transfected with (Lane 1) or without (Lane 2) Flag-tagged Bad using DYKDDDDK tag monoclonal antibody, clone G191 (Cat # MAB8183).Immunoprecipitation
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Publication Reference
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Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal.
Elliott TS, Townsley FM, Bianco A, Ernst RJ, Sachdeva A, Elsasser SJ, Davis L, Lang K, Pisa R, Greiss S, Lilley KS, Chin JW.
Nature Biotechnology 2014 May; 32(5):465.
Application：WB-Tr, Human, HEK293T cells.
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Production and purification of human indoleamine 2,3-dioxygenase (HuIDO) protein in a baculovirus expression system and production and characterization of egg yolk antibody against the purified HuIDO.
Webster NL, Wee J, Uren SJ, Boyle W, Sandrin MS.
Immunology and Cell Biology 2005 Oct; 83(5):542.
Application：WB-Tr, Insect cells, Sf21 insect cells.
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Proteome labeling and protein identification in specific tissues and at specific developmental stages in an animal.
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