Fto monoclonal antibody, clone 5-2H10
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Fto.
Immunogen
A synthetic peptide corresponding to N-terminus of rat Fto.
Host
Mouse
Theoretical MW (kDa)
58
Reactivity
Human, Mouse, Rat
Specificity
Specific for endogenous levels of the ~58 kDa FTO protein.
Form
Liquid
Purification
Protein G purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat testis lysate showing specific immunolabeling of the ~ 58 kDa Fto protein.Immunofluorescence
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Publication Reference
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Inactivation of the Fto gene protects from obesity.
Fischer J, Koch L, Emmerling C, Vierkotten J, Peters T, Bruning JC, Ruther U.
Nature 2009 Feb; 458(7240):894.
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The obesity gene, FTO, is of ancient origin, up-regulated during food deprivation and expressed in neurons of feeding-related nuclei of the brain.
Fredriksson R, Hagglund M, Olszewski PK, Stephansson O, Jacobsson JA, Olszewska AM, Levine AS, Lindblom J, Schioth HB.
Endocrinology 2008 May; 149(5):2062.
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Inactivation of the Fto gene protects from obesity.
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