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Last updated: 2022/5/22

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Actin monoclonal antibody, clone C4 

  • Catalog # : MAB8172
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native Actin.
  • Immunogen:
  • Native purified Actin from Chicken gizzard.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 42
  • Reactivity:
  • Chicken, Human, Mouse, Rat
  • Specificity:
  • Specific for endogenous levels of the ~42 kDa actin protein. Reacts with all actin isoforms.
  • Form:
  • Liquid
  • Purification:
  • Unpurified ascites fluid.
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunofluorescence (1:200-1:500)
    Immunohistochemistry (1:500)
    Western Blot (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites fluid (10 mM sodium azide).
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
    Recommended that the antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20°C or -80°C, while keeping a working aliquot stored at 4°C for short term.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein.
  • Immunohistochemistry
  • Immunofluorescence
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry
  • Immunofluorescence
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