Western blot using Ahsa1 monoclonal antibody, clone 25F2.D9 (Cat # MAB8169) shows detection of a band ~42 KDa in size corresponding to Ahsa1 in A-431 whole cell lysate (Lane 1) and MCF-7 whole cell lysate (Lane 2). A control lane is shown where primary ahntibody was omitted from the incubation (Lane 3). Molecular weight markers are shown at the left. For best results, block the membrane overnight with 3% BSA in TBS followed by reaction with primary antibody diluted 1 : 1,000 and use HRP conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer for detection.
Immunohistochemical staining of Ahsa1 monoclonal antibody, clone 25F2.D9 (Cat # MAB8169) was used at a 5-10 ug/mL to detect Ahsa1 in the seminiferous tubule of human testis (40X) showing moderate staining. Leydig cells showed faint to moderate staining. Expression of Ahsa1 is reported in many epithelial and lymphatic tissues, with cytoplasmic localization. This antibody showed moderate cytoplasmic staining of a variety of epithelial tissues and lymphoid organs such as spleen and tonsil with minimal background staining. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.