Stat2 monoclonal antibody, clone 19G8.H2.H6
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot from HeLa whole cell lysate was loaded at 1 ug. The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with Stat2 monoclonal antibody, clone 19G8.H2.H6 (Cat # MAB8168) in 3% BSA/TBST at 1 : 1,000 overnight at 4C. After washing, blot was incubated with Rockland Immunochemicals' HRP Rb a-Ms IgG antibody in blocking buffer for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of Stat2 monoclonal antibody, clone 19G8.H2.H6 (Cat # MAB8168) was used at a 2.5 ug/mL to detect Stat2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (image). Expression of STAT2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Vasiliki Demas, LifeSpan Biosciences, Seattle, WA.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of Stat2.
Immunogen
A synthetic peptide corresponding to C-terminus of mouse Stat2.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
ELISA (1:5000-1:10000)
Western Blot (1:1000)
Immunohistochemistry (2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot from HeLa whole cell lysate was loaded at 1 ug. The blot was blocked with 1% BSA in TBST for 30 min at RT, then washed and incubated with Stat2 monoclonal antibody, clone 19G8.H2.H6 (Cat # MAB8168) in 3% BSA/TBST at 1 : 1,000 overnight at 4C. After washing, blot was incubated with Rockland Immunochemicals' HRP Rb a-Ms IgG antibody in blocking buffer for 30 minutes at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of Stat2 monoclonal antibody, clone 19G8.H2.H6 (Cat # MAB8168) was used at a 2.5 ug/mL to detect Stat2 in squamous epithelium from human skin (40X) showing moderate to strong nuclear and faint to moderate cytoplasmic staining (image). Expression of STAT2 is expected to be cytoplasmic, and nuclear upon activation. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Vasiliki Demas, LifeSpan Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Stat2
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Publication Reference
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Functional subdomains of STAT2 required for preassociation with the alpha interferon receptor and for signaling.
Li X, Leung S, Kerr IM, Stark GR.
Molecular and Cellular Biology 1997 Apr; 17(4):2048.
Application：WB-Tr, Human, U6A cells.
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Functional subdomains of STAT2 required for preassociation with the alpha interferon receptor and for signaling.
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