TRPC6 monoclonal antibody, clone 3F2.H10.F2
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry using TRPC6 monoclonal antibody, clone 3F2.H10.F2 (Cat # MAB8167) shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 ug/mL . The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of TRPC6.
Immunogen
A synthetic peptide corresponding to C-terminus of human TRPC6 .
Host
Mouse
Reactivity
Chimpanzee, Human
Form
Liquid
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
Immunohistochemistry (2.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry using TRPC6 monoclonal antibody, clone 3F2.H10.F2 (Cat # MAB8167) shows detection of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 ug/mL . The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TRPC6
Entrez GeneID
7225Gene Name
TRPC6
Gene Alias
FLJ11098, FLJ14863, FSGS2, TRP6
Gene Description
transient receptor potential cation channel, subfamily C, member 6
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq
Other Designations
transient receptor potential channel 6
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Interactome
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Disease
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Publication Reference
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TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function.
Reiser J, Polu KR, Moller CC, Kenlan P, Altintas MM, Wei C, Faul C, Herbert S, Villegas I, Avila-Casado C, McGee M, Sugimoto H, Brown D, Kalluri R, Mundel P, Smith PL, Clapham DE, Pollak MR.
Nature Genetics 2005 Jul; 37(7):739.
Application：IEF, IF, IHC-Fr, IP, WB, Mouse Rat, Kidney glomerulus, Podocytes.
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Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol.
Hofmann T, Obukhov AG, Schaefer M, Harteneck C, Gudermann T, Schultz G.
Nature 1999 Jan; 397(6716):259.
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Identification and assignment of the human transient receptor potential channel 6 gene TRPC6 to chromosome 11q21-->q22.
D'Esposito M, Strazzullo M, Cuccurese M, Spalluto C, Rocchi M, D'Urso M, Ciccodicola A.
Cytogenetics and Cell Genetics 1998 Jan; 83(1-2):46.
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TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function.
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