LGR4 monoclonal antibody, clone 6G8.B3.G5.C3
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of LGR4 monoclonal antibody, clone 6G8.B3.G5.C3 (Cat # MAB8166) was used diluted to 5 ug/mL to detect LGR4 staining at the membrane of cells in various human tissues.
A. Brain cerebellum.
B. Pancreas islet.
Strongly positive staining is noted in subsets of cells within the islets of Langerhans. Moderately positive staiing was observed in Purkinje and Golgi neurons of the cerebellum, adrenal medulla, neuroendocrine cells, hepatocytes, lung macrophages, seminiferous tubules and Leydig cells of the testis. Faintly to moderately positive staining was also observed in cardiac myocytes and renal tubules, granulocytes, and subsets of lymphocytes. Some elastin background staining is noted. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of LGR4.
Immunogen
A synthetic peptide corresponding to internal region of human LGR4 .
Host
Mouse
Reactivity
Chimpanzee, Human, Macaque
Form
Liquid
Recommend Usage
ELISA (1:20000-1:100000)
Western Blot (1:500-1:3000)
Immunohistochemistry (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of LGR4 monoclonal antibody, clone 6G8.B3.G5.C3 (Cat # MAB8166) was used diluted to 5 ug/mL to detect LGR4 staining at the membrane of cells in various human tissues.
A. Brain cerebellum.
B. Pancreas islet.
Strongly positive staining is noted in subsets of cells within the islets of Langerhans. Moderately positive staiing was observed in Purkinje and Golgi neurons of the cerebellum, adrenal medulla, neuroendocrine cells, hepatocytes, lung macrophages, seminiferous tubules and Leydig cells of the testis. Faintly to moderately positive staining was also observed in cardiac myocytes and renal tubules, granulocytes, and subsets of lymphocytes. Some elastin background staining is noted. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal communication, Andrew Elston, Lifespan Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — LGR4
Entrez GeneID
55366Gene Name
LGR4
Gene Alias
GPR48
Gene Description
leucine-rich repeat-containing G protein-coupled receptor 4
Omim ID
606666Gene Ontology
HyperlinkGene Summary
G protein-coupled receptors (GPCRs) play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain (Weng et al., 2008 [PubMed 18424556]).[supplied by OMIM
Other Designations
G protein-coupled receptor 48|G-protein-coupled receptor 48
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Interactome
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Pathway
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Publication Reference
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Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2.
Weng J, Luo J, Cheng X, Jin C, Zhou X, Qu J, Tu L, Ai D, Li D, Wang J, Martin JF, Amendt BA, Liu M.
PNAS 2008 Apr; 105(16):6081.
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Up-regulation of GPR48 induced by down-regulation of p27Kip1 enhances carcinoma cell invasiveness and metastasis.
Gao Y, Kitagawa K, Hiramatsu Y, Kikuchi H, Isobe T, Shimada M, Uchida C, Hattori T, Oda T, Nakayama K, Nakayama KI, Tanaka T, Konno H, Kitagawa M.
Cancer Research 2006 Dec; 66(24):11623.
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Molecular characterization of a novel glycoprotein hormone G-protein-coupled receptor.
Loh ED, Broussard SR, Kolakowski LF.
Biochemical and Biophysical Research Communications 2001 Apr; 282(3):757.
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Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2.
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