GDF15 monoclonal antibody, clone 7C12.B3.F2
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More Files
- More Functions
Western Blot
Western blot using GDF15 monoclonal antibody, clone 7C12.B3.F2 (Cat # MAB8162).
The blot shows detection of recombinant GDF15 protein present in Pichia pastoris whole cell lysates.
Lane 1, yeast cell lysate expressing GDF15 H variant with SUMO expression tag at 36 KDa
Lane 2, yeast cell lysate expressing GDF15 D variant with SUMO expression tag at 36 KDa.
Lane 3, yeast cell lysate expressing GDF15 H variant.
Lane 4, yeast cell lysate expressing GDF15 D variant.
All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of GDF15.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus end of human GDF15 .
Host
Mouse
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:150000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using GDF15 monoclonal antibody, clone 7C12.B3.F2 (Cat # MAB8162).
The blot shows detection of recombinant GDF15 protein present in Pichia pastoris whole cell lysates.
Lane 1, yeast cell lysate expressing GDF15 H variant with SUMO expression tag at 36 KDa
Lane 2, yeast cell lysate expressing GDF15 D variant with SUMO expression tag at 36 KDa.
Lane 3, yeast cell lysate expressing GDF15 H variant.
Lane 4, yeast cell lysate expressing GDF15 D variant.
All lysates were run under reducing conditions. Primary antibody was used at a 1 : 1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the BioRad Versadoc™ 4000MP Imaging System.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GDF15
Entrez GeneID
9518Gene Name
GDF15
Gene Alias
GDF-15, MIC-1, MIC1, NAG-1, PDF, PLAB, PTGFB
Gene Description
growth differentiation factor 15
Omim ID
605312Gene Ontology
HyperlinkGene Summary
Bone morphogenetic proteins (e.g., BMP5; MIM 112265) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM
Other Designations
NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1|PTGF-beta|prostate differentiation factor
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Interactome
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Disease
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Publication Reference
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Changes in gene expression contribute to cancer prevention by COX inhibitors.
Baek SJ, Eling TE.
Progress in Lipid Research 2005 Nov; 45(1):1.
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H6D polymorphism in macrophage-inhibitory cytokine-1 gene associated with prostate cancer.
Lindmark F, Zheng SL, Wiklund F, Bensen J, Balter KA, Chang B, Hedelin M, Clark J, Stattin P, Meyers DA, Adami HO, Isaacs W, Gronberg H, Xu J.
Journal of the National Cancer Institute 2004 Aug; 96(16):1248.
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Changes in gene expression contribute to cancer prevention by COX inhibitors.
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