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Last updated: 2016/12/4
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GDF15 monoclonal antibody, clone 23B3.D2.H5

  • Catalog # : MAB8161
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of GDF15 .
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus end of human GDF15 .
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein A chromatography
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:2000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Western Blot
  • Western blot using GDF15 monoclonal antibody, clone 23B3D2.H5 (Cat # MAB8161).
    The blot shows detection of recombinant GDF15 protein present in Pichia pastoris whole cell lysates.
    Lane 1, yeast cell lysate expressing GDF15 H variant with SUMO expression tag at 36 KDa.
    Lane 2, yeast cell lysate expressing GDF15 D variant with SUMO expression tag at 36 KDa.
    Lane 3, yeast cell lysate expressing GDF15 H variant.
    Lane 4, yeast cell lysate expressing GDF15 D variant.
    Recombinant GDF15 proteins without SUMO correspond to monomer (15 KDa) and dimer (30 KDa) bands as indicated by the arrowheads. All lysates were run under reducing conditions.
    Primary antibody was used at a 1 : 1,000 dilution in TBS containg 1% BSA and 0.2% Tween, and reacted overnight at 4°C. For detection, a 1 : 40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimati
  • ELISA
  • Application Image
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9518
  • Gene Name:
  • GDF15
  • Gene Alias:
  • GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB
  • Gene Description:
  • growth differentiation factor 15
  • Gene Summary:
  • Bone morphogenetic proteins (e.g., BMP5; MIM 112265) are members of the transforming growth factor-beta (see TGFB1; MIM 190180) superfamily and regulate tissue differentiation and maintenance. They are synthesized as precursor molecules that are processed at a dibasic cleavage site to release C-terminal domains containing a characteristic motif of 7 conserved cysteines in the mature protein.[supplied by OMIM
  • Other Designations:
  • NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1,PTGF-beta,prostate differentiation factor
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