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Last updated: 2016/10/23
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WNT1 monoclonal antibody, clone 13F9

  • Catalog # : MAB8160
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of WNT1 .
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human WNT1 .
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • ELISA (1:4000-1:20000)
    Western Blot (1:175-1:250)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using WNT1 monoclonal antibody, clone 13F9 (Cat # MAB8160) shows detection of WNT1 protein in mouse testis lysate. The results show specific binding corresponding to the ~41 KDa WNT1 protein. Primary antibody was used at a 1 : 500 dilution.
    Personal communication, Stephen Brown, Brown University.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7471
  • Gene Name:
  • WNT1
  • Gene Alias:
  • INT1
  • Gene Description:
  • wingless-type MMTV integration site family, member 1
  • Gene Summary:
  • The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq
  • Other Designations:
  • Wingless-type MMTV integration site family, member 1 (oncogene INT1)
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