Western Blotting from HeLa whole cell lysate (Lane 1) and HEK293 whole cell lysate (Lane 2) were loaded with 10 ug of lysate each. TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) at 1 : 1,000 for 1 h at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.
Immunohistochemical staining of TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) was used at a 2.5 ug/mL to detect TUBA1B in human thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.
Immunofluorescence staining of TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) was used at a 0.1 ug/mL to detect TUBA1B in 4% paraformaldehyde fixed A-549 cells. Staining is shown using conventional confocal microscopy (upper panel) and by high resolution TCS STED nanoscopy (bottom panel). DyLight488™ conjugated anti-mouse IgG secondary antibody was used for detection at 1 ug/mL . Personal Communication, Myriam. Gastard, Leica Microsystems, Exton PA.