TUBA1B monoclonal antibody, clone 17H11.F10
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More Files
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Western Blot (Cell lysate)
Western Blotting from HeLa whole cell lysate (Lane 1) and HEK293 whole cell lysate (Lane 2) were loaded with 10 ug of lysate each. TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) at 1 : 1,000 for 1 h at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) was used at a 2.5 ug/mL to detect TUBA1B in human thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.
Immunofluorescence
Immunofluorescence staining of TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) was used at a 0.1 ug/mL to detect TUBA1B in 4% paraformaldehyde fixed A-549 cells. Staining is shown using conventional confocal microscopy (upper panel) and by high resolution TCS STED nanoscopy (bottom panel). DyLight488™ conjugated anti-mouse IgG secondary antibody was used for detection at 1 ug/mL .
Personal Communication, Myriam. Gastard, Leica Microsystems, Exton PA.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of TUBA1B.
Immunogen
A synthetic peptide corresponding to C-terminus end of human TUBA1B.
Host
Mouse
Reactivity
Bovine, Chicken, Human, Mouse, Rat
Specificity
Cross reactivity will occur with all isoforms of alpha tubulin.
Form
Liquid
Recommend Usage
ELISA (1:300000)
Western Blot (1:1000)
Immunohistochemistry (2.5 ug/mL)
Immunofluorescence (0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting from HeLa whole cell lysate (Lane 1) and HEK293 whole cell lysate (Lane 2) were loaded with 10 ug of lysate each. TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) at 1 : 1,000 for 1 h at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) was used at a 2.5 ug/mL to detect TUBA1B in human thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Vasiliki Demas, LifeSpanBiosciences, Seattle, WA.Immunofluorescence
Immunofluorescence staining of TUBA1B monoclonal antibody, clone 17H11.F10 (Cat # MAB8159) was used at a 0.1 ug/mL to detect TUBA1B in 4% paraformaldehyde fixed A-549 cells. Staining is shown using conventional confocal microscopy (upper panel) and by high resolution TCS STED nanoscopy (bottom panel). DyLight488™ conjugated anti-mouse IgG secondary antibody was used for detection at 1 ug/mL .
Personal Communication, Myriam. Gastard, Leica Microsystems, Exton PA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TUBA1B
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Interactome
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Pathway
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Publication Reference
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Structural features and restricted expression of a human alpha-tubulin gene.
Hall JL, Cowan NJ.
Nucleic Acids Research 1985 Jan; 13(1):207.
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Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.
Cowan NJ, Dobner PR, Fuchs EV, Cleveland DW.
Molecular and Cellular Biology 1993 Oct; 3(10):1738.
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Structural features and restricted expression of a human alpha-tubulin gene.
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