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Last updated: 2017/12/17
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Kcnc4 monoclonal antibody, clone S72-16

  • Catalog # : MAB7897
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic peptide of Dlg2.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 175-192 of rat Dlg2.
  • Sequence:
  • GDEAGDDERELALQRLGP
  • Host:
  • Mouse
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • Detects ~70k Da (100 kDa in brain due to glycosylation).
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Immunocytochemistry (1:100)
    Immunofluorescence (1:100)
    Immunohistochemistry (1:1000)
    Immunoprecipitation
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western Blot analysis of rat brain membrane lysate with Kcnc4 monoclonal antibody, clone S72-16 (Cat # MAB7897) at 1:1000 for 2 hours at RT. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of mouse backskin with Kcnc4 monoclonal antibody, clone S72-16 (Cat # MAB7897) at 1:100 for 1 hour at RT. Fixation: Bouin's Fixative and paraffin-embedded. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hints of filaggrinlike staining.
  • Immunohistochemistry
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining of HaCaT cells with Kcnc4 monoclonal antibody, clone S72-16 (Cat # MAB7897) at 1:100 for 1 hour at RT. Fixation: Cold 100% methanol for 10 minutes at -20°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive, stains nucleoli.
  • Immunofluorescence
  • Immunoprecipitation
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Gene Information
  • Gene Name:
  • Kcnc4
  • Gene Alias:
  • -
  • Gene Description:
  • potassium voltage gated channel, Shaw-related subfamily, member 4
  • Other Designations:
  • potassium voltage-gated channel subfamily C member 4 (Voltage-gated potassium channel subunit Kv3.4) (Raw3),voltage-gated potassium channel
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