Shank1 monoclonal antibody, clone S22-21
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More Files
- More Functions
Western Blot (Tissue lysate)
Western Blot analysis of rat brain membrane lysate.
Immunohistochemistry
Immunohistochemical staining of mouse backskin with Shank1 monoclonal antibody, clone S22-21 at 1:100. Fixation: Bouin's Fixative and paraffin-embedded. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50. Localization: Filaggrinlike staining (upper layer aggregations of staining).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Shank1.
Immunogen
Recombinant protein corresponding to amino acids 469-691 of rat Shank1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
Detects ~190-220 kDa (alternative splice variants). No cross-reactivity against Shank2 or Shank3.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
Immunocytochemistry (1:100)
Immunofluorescence (1:100)
Immunohistochemistry (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western Blot analysis of rat brain membrane lysate.Immunohistochemistry
Immunohistochemical staining of mouse backskin with Shank1 monoclonal antibody, clone S22-21 at 1:100. Fixation: Bouin's Fixative and paraffin-embedded. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50. Localization: Filaggrinlike staining (upper layer aggregations of staining).Immunocytochemistry
Immunofluorescence
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Gene Info — Shank1
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Publication Reference
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Smaller dendritic spines, weaker synaptic transmission, but enhanced spatial learning in mice lacking Shank1.
Hung AY, Futai K, Sala C, Valtschanoff JG, Ryu J, Woodworth MA, Kidd FL, Sung CC, Miyakawa T, Bear MF, Weinberg RJ, Sheng M.
Journal of Neuroscience 2008 Feb; 28(7):1697.
Application：IF, WB-Ti, Mouse, Mouse brain.
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The Shank family of scaffold proteins.
Sheng M, Kim E.
Journal of Cell Science 2000 Jun; 113 ( Pt 11):1851.
Application：IHC, WB, Human, Human brains.
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Smaller dendritic spines, weaker synaptic transmission, but enhanced spatial learning in mice lacking Shank1.
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