Cell lysates of 293T (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with UBE2S monoclonal antibody, clone AT2C12 (Cat # MAB7885) (1 : 250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and UBE2S monoclonal antibody, clone AT2C12 (Cat # MAB7885) (1 : 500) with Alexa 488 (Green).
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq
ubiquitin carrier protein S,ubiquitin-conjugating enzyme E2-24 kD,ubiquitin-protein ligase S