XBP1 monoclonal antibody, clone 9B7E5
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of 100 ng of XBP1 recombinant protein with XBP1 monoclonal antibody, clone 9B7E5 (Cat # MAB7874) at 1 ug/mL .
Immunocytochemistry
Immunocytochemistry of XBP1 in HepG2 cells with XBP1 monoclonal antibody, clone 9B7E5 (Cat # MAB7874) at 2 ug/mL .
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant XBP1.
Immunogen
Recombinant protein corresponding to amino acids 2-160 of human XBP1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of 100 ng of XBP1 recombinant protein with XBP1 monoclonal antibody, clone 9B7E5 (Cat # MAB7874) at 1 ug/mL .Immunocytochemistry
Immunocytochemistry of XBP1 in HepG2 cells with XBP1 monoclonal antibody, clone 9B7E5 (Cat # MAB7874) at 2 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — XBP1
Entrez GeneID
7494Protein Accession#
BAB82982Gene Name
XBP1
Gene Alias
TREB5, XBP2
Gene Description
X-box binding protein 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq
Other Designations
OTTHUMP00000028682|X-box-binding protein-1
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Interactome
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Disease
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Publication Reference
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IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA.
Calfon M, Zeng H, Urano F, Till JH, Hubbard SR, Harding HP, Clark SG, Ron D.
Nature 2002 Jan; 415(6867):92.
Application：WB, Mouse , MEF, Mouse fibroblasts.
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XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor.
Yoshida H, Matsui T, Yamamoto A, Okada T, Mori K.
Cell 2001 Dec; 107(7):881.
Application：WB-Ce, WB-Tr, Human, HeLa cells.
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IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA.
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