Mouse monoclonal antibody raised against recombinant Simian immunodeficiency virus type 1 Nef.
Recombinant protein corresponding to Simian immunodeficiency virus type 1 (mac251 strain) Nef.
Mapped to amino acids 71-80 (-PAAEREKLAY-, SIV-1). Reacts in ELISA, WB, RIP, IHC with Nef of SIVmac251 strain (Piguet 1998, Khan 1998, Guillemin 2000). Reacts in IF with Nef of SIVmac239 strain and SIVmac1A11 strain (Khan 1998, Chan 2005).
Protein G purification
ELISA (0.05-0.1 ug/mL) Immunohistochemistry Immunoprecipitation Western Blot (1 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.