Human immunodeficiency virus type 1 Nef monoclonal antibody, clone JR6
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human immunodeficiency virus type 1 Nef.
Immunogen
Recombinant protein corresponding to human immunodeficiency virus type 1 (B subtype consensus) Nef.
Host
Mouse
Reactivity
Viruses
Specificity
Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus). This antibody reacts with HIV-1 Nef subtypes A, B and C; JR-CSF, BRU, IIIB, LAV, BH10 strains.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1
Recommend Usage
ELISA (0.02-0.1 ug/mL)
Western Blot (0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines.
Kjerrstrom A, Hinkula J, Engstrom G, Ovod V, Krohn K, Benthin R, Wahren B.
Virology 2001 May; 284(1):46.
Application:WB-Tr, Human, HeLa cells.
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Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors.
Collings A, Pitkanen J, Strengell M, Tahtinen M, Lagerstedt A, Hakkarainen K, Ovod V, Sutter G, Ustav M, Ustav E, Mannik A, Ranki A, Peterson P, Krohn K.
Vaccine 1999 Oct; 18(5-6):460.
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Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia.
Ranki A, Nyberg M, Ovod V, Haltia M, Elovaara I, Raininko R, Haapasalo H, Krohn K.
AIDS 1995 Sep; 9(9):1001.
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Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines.
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