CRABP1 monoclonal antibody, clone AT1A1
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mouse eye (30 ug) tissue lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with CRABP1 monoclonal antibody, clone AT1A1 (Cat # MAB7558) (1 : 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: CRABP1 Transfected 293T cell lysate.
Immunofluorescence
Immunofluorescence analysis of Balb/3T3 cells. The cell was stained with CRABP1 monoclonal antibody, clone AT1A1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometry analysis of CRABP1 in Balb/3T3 cell line, staining at 2-5 ug for 1x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised aganist full length recombinant CRABP1.
Immunogen
Recombinant protein corresponding to full length human CRABP1.
Host
Mouse
Reactivity
Human, Mouse
Specificity
This antibody reacts with human CRABP1.
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse eye (30 ug) tissue lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with CRABP1 monoclonal antibody, clone AT1A1 (Cat # MAB7558) (1 : 500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: CRABP1 Transfected 293T cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Balb/3T3 cells. The cell was stained with CRABP1 monoclonal antibody, clone AT1A1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of CRABP1 in Balb/3T3 cell line, staining at 2-5 ug for 1x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — CRABP1
Entrez GeneID
1381Protein Accession#
NP_004369Gene Name
CRABP1
Gene Alias
CRABP, CRABP-I, CRABPI, RBP5
Gene Description
cellular retinoic acid binding protein 1
Omim ID
180230Gene Ontology
HyperlinkGene Summary
This gene encodes a specific binding protein for a vitamin A family member and is thought to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid at specific sites within the nucleus, which may contribute to vitamin A-directed differentiation in epithelial tissue. [provided by RefSeq
Other Designations
cellular retinoic acid-binding protein 1
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Interactome
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Disease
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Publication Reference
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Molecular and metabolic retinoid pathways in the human ocular surface.
Nezzar H, Chiambaretta F, Marceau G, Blanchon L, Faye B, Dechelotte P, Rigal D, Sapin V.
Molecular Vision 2007 Sep; 13:1641.
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Structure-function relationships of cellular retinoic acid-binding proteins. Quantitative analysis of the ligand binding properties of the wild-type proteins and site-directed mutants.
Wang L, Li Y, Yan H.
The Journal of Biological Chemistry 1997 Jan; 272(3):1541.
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Molecular and metabolic retinoid pathways in the human ocular surface.
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