Cell lysates of HepG2 (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with CBR3 monoclonal antibody, clone AT3C5 (Cat # MAB7557) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq
NADPH-dependent carbonyl reductase 3,carbonyl reductase (NADPH) 3,short chain dehydrogenase/reductase family 21C, member 2