Cell lysates of HeLa (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with BRCC3 monoclonal antibody, clone AT3B1 (Cat # MAB7555) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint. Alternative splicing results in multiple transcript variants. [provided by RefSeq