Mouse monoclonal antibody raised aganist full length recombinant BRCC3.
Immunogen:
Recombinant protein corresponding to full length human BRCC3.
Host:
Mouse
Reactivity:
Human
Specificity:
This antibody reacted with human BRCC3.
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG1, kappa
Recommend Usage:
ELISA Flow Cytometry Immunohistochemistry Immunocytochemistry Immunofluorescence Western Blot The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: 293T cell lysate, Lane 4: MCF7 cell lysate, Lane 5: NIH3T3 cell lysate.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with BRCC3 monoclonal antibody, clone AT3B1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint. Alternative splicing results in multiple transcript variants. [provided by RefSeq