BRCC3 monoclonal antibody, clone AT3B1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: 293T cell lysate, Lane 4: MCF7 cell lysate, Lane 5: NIH3T3 cell lysate.
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with BRCC3 monoclonal antibody, clone AT3B1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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Specification
Product Description
Mouse monoclonal antibody raised aganist full length recombinant BRCC3.
Immunogen
Recombinant protein corresponding to full length human BRCC3.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacted with human BRCC3.
Form
Liquid
Purification
Protein G purification
Isotype
IgG1, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HeLa cell lysate, Lane 2: HepG2 cell lysate, Lane 3: 293T cell lysate, Lane 4: MCF7 cell lysate, Lane 5: NIH3T3 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with BRCC3 monoclonal antibody, clone AT3B1 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — BRCC3
Entrez GeneID
79184Protein Accession#
NP_077308Gene Name
BRCC3
Gene Alias
BRCC36, C6.1A, CXorf53
Gene Description
BRCA1/BRCA2-containing complex, subunit 3
Omim ID
300617Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRCA1/BRCA2-containing complex subunit 36|OTTHUMP00000061450|c6.1A
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Interactome
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Publication Reference
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Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage.
Wang B, Elledge SJ.
PNAS 2007 Dec; 104(52):20759.
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BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation.
Chen X, Arciero CA, Wang C, Broccoli D, Godwin AK.
Cancer Research 2006 May; 66(10):5039.
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Regulation of BRCC, a holoenzyme complex containing BRCA1 and BRCA2, by a signalosome-like subunit and its role in DNA repair.
Dong Y, Hakimi MA, Chen X, Kumaraswamy E, Cooch NS, Godwin AK, Shiekhattar R.
Molecular Cell 2003 Nov; 12(5):1087.
Application：WB-Tr, Human, HEK 293 cells.
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Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage.
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