DYKDDDDK tag monoclonal antibody, clone L5
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More Files
- More Functions
Western Blot
Western blot on DYKDDDDK tagged protein demonstrating that the DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG® M2 antibody.
Western Blot (Recombinant protein)
Western blot on DYKDDDDK tagged protein demonstrating that the DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG® M2 antibody.
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Specification
Product Description
Rat monoclonal antibody raised against DYKDDDDK tag.
Immunogen
N-terminal DYKDDDDK-tagged extracellular domain of of mouse Langerin.
Host
Rat
Reactivity
Human, Mouse, Rat
Specificity
DYKDDDDK Epitope Tag (L5).
Form
Liquid
Isotype
IgG2a
Recommend Usage
Immunofluorescence (1:10)
Western Blot (1:1000)
Immunocytochemistry (1:10)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot on DYKDDDDK tagged protein demonstrating that the DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG® M2 antibody.Western Blot (Recombinant protein)
Western blot on DYKDDDDK tagged protein demonstrating that the DYKDDDDK tag monoclonal antibody, clone L5 (Cat # MAB7373) is 10-15 fold more sensitive than Sigma's M2 mouse ANTI-FLAG® M2 antibody.Immunocytochemistry
Immunofluorescence
Immunoprecipitation
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Publication Reference
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Momordica anti-HIV protein MAP30 abrogates the Epstein-Barr virus nuclear antigen 1 dependent functions in host cells.
Wei-Hang Huang, Wen-Min Su, Chung-Wei Wang, Yue-Hao Fang, Yuan-Wei Jian, Hao-Jen Hsu, Chih-Wen Peng.
Heliyon 2023 Oct; 9(11):e21486.
Application：WB, Human, BJAB cells.
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Cutting edge: langerin/CD207 receptor on dendritic cells mediates efficient antigen presentation on MHC I and II products in vivo.
Idoyaga J, Cheong C, Suda K, Suda N, Kim JY, Lee H, Park CG, Steinman RM.
Journal of Immunology 2008 Mar; 180(6):3647.
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Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection.
Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG.
Journal of Immunological Methods 2008 Feb; 331(1-2):27.
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Momordica anti-HIV protein MAP30 abrogates the Epstein-Barr virus nuclear antigen 1 dependent functions in host cells.
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