ALPP monoclonal antibody, clone 8B6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against native ALPP.
Immunogen
Hep-2 cells with boosted surface expression of alkaline phosphatase, placental.
Host
Mouse
Theoretical MW (kDa)
70
Reactivity
Human
Specificity
Alkaline phosphatase, placental - both Regan and Nagao isoenzymes. No cross reactivity with other isoenzymes of alkaline phosphatase.
Form
Liquid
Purification
Protein A purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA (1:100-1:2000)
Flow Cytometry
Immunocytochemistry/Immunofluorescence (1:10-1:500)
Immunohistochemistry (1:10-1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100-1:200)
Radioimmunodiffusion
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-Glycine, 0.15 M NaCl (0.05% sodium azide).
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Frozen sections)
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Immunodiffusion
-
Gene Info — ALPP
Entrez GeneID
250Protein Accession#
P05187Gene Name
ALPP
Gene Alias
ALP, FLJ61142, PALP, PLAP
Gene Description
alkaline phosphatase, placental (Regan isozyme)
Omim ID
171800Gene Ontology
HyperlinkGene Summary
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized. [provided by RefSeq
Other Designations
alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
RhoB/ROCK mediates oxygen-glucose deprivation-stimulated syncytiotrophoblast microparticle shedding in preeclampsia.
Han J, Yang BP, Li YL, Li HM, Zheng XH, Yu LL, Zhang Q, Zheng YR, Yi P, Li L, Guo JX, Zhou YG.
Cell and Tissue Research 2016 Jun; 366(2):411.
Application：WB, Human, Placenta tissue.
-
Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.
Durbin H, Milligan EM, Mather SJ, Tucker DF, Raymond R, Bodmer WF.
International Journal of Cancer 1988 Jan; 2:59.
Application：Injection, Recombinant protein.
-
Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations.
Durbin H, Tucker DF, Milligan EM, Bobrow LG, Warne PH, Pookim YL, Bodmer WF.
International Journal of Cancer 1988 Jan; 2:50.
Application：IHC-P, ELISA, Human, Ovary, Seminoma, Gastric adenocarcinoma, Serum.
-
RhoB/ROCK mediates oxygen-glucose deprivation-stimulated syncytiotrophoblast microparticle shedding in preeclampsia.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com