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Last updated: 2016/12/4
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GLO1 monoclonal antibody, clone Glo1a

  • Catalog # : MAB7193
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against full length recombinant GLO1.
  • Immunogen:
  • Recombinant GST fusion protein corresponding to full length human GLO1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • GLO1 (Glo1a). A band is seen ~21 KDa in WB.
  • Form:
  • Liquid
  • Isotype:
  • IgG1, kappa
  • Recommend Usage:
  • ELISA (50 ug/1 ug protein)
    Immunofluorescence (10 ug/mL)
    Western Blot (0.5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot on HeLa whole cell extracts with GLO1 monoclonal antibody, clone Glo1a (Cat # MAB7193).
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunostaining of human retinal endothelial cells.
    Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with GLO1 monoclonal antibody, clone Glo1a (Cat # MAB7193) using anti mouse IgG Texas Red as secondary Ab.
    The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain.
    No reactivity was detected in control cells, stained without Go1a mAb.
    Figure is a representative of 3 independent experiments.
    Photo courtesy of Dr. Nagaraj, Case Western Reserve University.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2739
  • Gene Name:
  • GLO1
  • Gene Alias:
  • GLOD1,GLYI
  • Gene Description:
  • glyoxalase I
  • Gene Summary:
  • The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000016339,glyoxalase domain containing 1,lactoyl glutathione lyase,lactoylglutathione lyase
  • Gene Pathway
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