GLO1 monoclonal antibody, clone Glo1a
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot on HeLa whole cell extracts with GLO1 monoclonal antibody, clone Glo1a (Cat # MAB7193).
Immunofluorescence
Immunostaining of human retinal endothelial cells.
Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with GLO1 monoclonal antibody, clone Glo1a (Cat # MAB7193) using anti mouse IgG Texas Red as secondary Ab.
The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain.
No reactivity was detected in control cells, stained without Go1a mAb.
Figure is a representative of 3 independent experiments.
Photo courtesy of Dr. Nagaraj, Case Western Reserve University.
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant GLO1.
Immunogen
Recombinant GST fusion protein corresponding to full length human GLO1.
Host
Mouse
Reactivity
Human
Specificity
GLO1 (Glo1a). A band is seen ~21 KDa in WB.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
ELISA (50 ug/1 ug protein)
Immunofluorescence (10 ug/mL)
Western Blot (0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot on HeLa whole cell extracts with GLO1 monoclonal antibody, clone Glo1a (Cat # MAB7193).Immunocytochemistry
Immunofluorescence
Immunostaining of human retinal endothelial cells.
Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells stained with GLO1 monoclonal antibody, clone Glo1a (Cat # MAB7193) using anti mouse IgG Texas Red as secondary Ab.
The cells were counter stained with phalloidin (green) and DAPI (blue), nuclear stain.
No reactivity was detected in control cells, stained without Go1a mAb.
Figure is a representative of 3 independent experiments.
Photo courtesy of Dr. Nagaraj, Case Western Reserve University.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GLO1
Entrez GeneID
2739GeneBank Accession#
Q04760Gene Name
GLO1
Gene Alias
GLOD1, GLYI
Gene Description
glyoxalase I
Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq
Other Designations
OTTHUMP00000016339|glyoxalase domain containing 1|lactoyl glutathione lyase|lactoylglutathione lyase
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Interactome
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Pathway
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Disease
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