CSPG4 monoclonal antibody, clone LHM 2
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of CSPG4 in human brain using DAB with hematoxylin counterstain
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Specification
Product Description
Mouse monoclonal antibody raised against native CSPG4.
Immunogen
Native purified CSPG4 from A375P cell crude extract.
Host
Mouse
Reactivity
Human
Specificity
MCSP (LHM 2). Predicted molecular weight 240 KDa.
Form
Liquid
Concentration
1.0 mg/mL
Isotype
IgG1, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In Tris-glycine, 150 mM NaCl (0.05 sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of CSPG4 in human brain using DAB with hematoxylin counterstainImmunoprecipitation
Flow Cytometry
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Gene Info — CSPG4
Entrez GeneID
1464Gene Name
CSPG4
Gene Alias
HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Gene Description
chondroitin sulfate proteoglycan 4
Omim ID
601172Gene Ontology
HyperlinkGene Summary
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq
Other Designations
chondroitin sulfate proteoglycan 4 (melanoma-associated)|melanoma-associated chondroitin sulfate proteoglycan
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Interactome
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Disease
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Publication Reference
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Isolation of human tumor-specific antibodies by selection of an antibody phage library on melanoma cells.
Kupsch JM, Tidman NH, Kang NV, Truman H, Hamilton S, Patel N, Newton Bishop JA, Leigh IM, Crowe JS.
Clinical Cancer Research 1999 Apr; 5(4):925.
Application：IF, IHC-Fr, Human, Human metastatic melanoma.
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Generation and selection of monoclonal antibodies, single-chain Fv and antibody fusion phage specific for human melanoma-associated antigens.
Kupsch JM, Tidman N, Bishop JA, McKay I, Leigh I, Crowe JS.
Melanoma Research 1995 Dec; 5(6):403.
Application：ELISA, Flow Cyt, IF, IHC-Fr, IP, Human, Bacteria, A-375P cells, Bacteria, DX3 cells, HMB2 cells, Human melanoma, Human non-skin tissues, Phage lysates, SK23 cells.
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Isolation of human tumor-specific antibodies by selection of an antibody phage library on melanoma cells.
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