PECAM1 monoclonal antibody, clone 158-2B3
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More Files
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Flow Cytometry
5 X 105 cultured human Jurkat cells were incubated 45 minutes on ice with 80 ml of PECAM1 monoclonal antibody, clone 158-2B3 (Cat # MAB6952) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.62 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
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Specification
Product Description
Mouse monoclonal antibody raised against native PECAM1.
Immunogen
Native purified PECAM1 from stimulated human leukocytes.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
5 X 105 cultured human Jurkat cells were incubated 45 minutes on ice with 80 ml of PECAM1 monoclonal antibody, clone 158-2B3 (Cat # MAB6952) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.62 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. -
Gene Info — PECAM1
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Interactome
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Pathway
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Disease
- Acute Disease
- Angina Pectoris
- Arteriosclerosis
- Asthma
- Astrocytoma
- Atherosclerosis
- Brain Ischemia
- Bronchiolitis
- Cadaver
+ View More Disease
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Publication Reference
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Transmigration of CD34+ cells across specialized and nonspecialized endothelium requires prior activation by growth factors and is mediated by PECAM-1 (CD31).
Yong KL, Watts M, Shaun Thomas N, Sullivan A, Ings S, Linch DC.
Blood 1998 Feb; 94(4):1196.
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The effect of intravenous administration of a chimeric anti-IgE antibody on serum IgE levels in atopic subjects: efficacy, safety, and pharmacokinetics.
Corne J, Djukanovic R, Thomas L, Warner J, Botta L, Grandordy B, Gygax D, Heusser C, Patalano F, Richardson W, Kilchherr E, Staehelin T, Davis F, Gordon W, Sun L, Liou R, Wang G, Chang TW, Holgate S.
The Journal of Clinical Investigation 1997 Mar; 99(5):879.
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Transmigration of CD34+ cells across specialized and nonspecialized endothelium requires prior activation by growth factors and is mediated by PECAM-1 (CD31).
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