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Flow Cytometry

Ficoll prepared human peripheral white blood cells were stimulated by incubating 1 day at 5 x 10⁶ cells/mL in RPMI 10% FBS media including 5 ug/mL Phytohemagglutinin-P. 5 X 10⁵ cells per tube were then washed and incubated 45 minutes on ice with 80 uL of KLRD1 monoclonal antibody, clone HP-3D9 (Cat # MAB6947) at a concentration of 20 ug/mL. Cells were washed twice and incubated with 2oreagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 40% population of the cells stained positive with a mean shift of 1.10 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.

Entrez GeneID

3824

Gene Name

KLRD1

Gene Alias

CD94

Gene Description

killer cell lectin-like receptor subfamily D, member 1

Omim ID

602894

Gene Ontology

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Gene Summary

Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq

Other Designations

CD94 antigen