ICAM3 monoclonal antibody, clone 186-2G9
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Specification
Product Description
Mouse monoclonal antibody raised against native ICAM3.
Immunogen
Native purified ICAM3 from stimulated human leukocytes.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2b, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
5 X 105 cultured human Jurkat cells per tube were washed and preincubated ~5 minutes on ice with 20 ml of 250 mg/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ml of ICAM3 monoclonal antibody, clone 186-2G9 (Cat # MAB6936) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. -
Gene Info — ICAM3
Entrez GeneID
3385Gene Name
ICAM3
Gene Alias
CD50, CDW50, ICAM-R
Gene Description
intercellular adhesion molecule 3
Omim ID
146631Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq
Other Designations
intercellular adhesion molecule-3
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Interactome
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Pathway
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Disease
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Publication Reference
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Critical amino acids in the lymphocyte function-associated antigen-1 I domain mediate intercellular adhesion molecule 3 binding and immune function.
van Kooyk Y, Binnerts ME, Edwards CP, Champe M, Berman PW, Figdor CG, Bodary SC.
The Journal of Experimental Medicine 1996 Mar; 183(3):1247.
Application:Func, Human, Human lymphocytes.
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Analysis of the binding site on intercellular adhesion molecule 3 for the leukocyte integrin lymphocyte function-associated antigen 1.
Holness CL, Bates PA, Little AJ, Buckley CD, McDowall A, Bossy D, Hogg N, Simmons DL.
The Journal of Biological Chemistry 1995 Jan; 270(2):877.
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Critical amino acids in the lymphocyte function-associated antigen-1 I domain mediate intercellular adhesion molecule 3 binding and immune function.
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