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Last updated: 2016/10/23
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ICAM3 monoclonal antibody, clone 186-2G9

  • Catalog # : MAB6936
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native ICAM3.
  • Immunogen:
  • Native purified ICAM3 from stimulated human leukocytes.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
  • Storage Instruction:
  • Store at 4°C.
  • Publication Reference
  • Applications
  • Flow Cytometry
  • Flow Cytometry
  • 5 X 105 cultured human Jurkat cells per tube were washed and preincubated ~5 minutes on ice with 20 ml of 250 mg/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ml of ICAM3 monoclonal antibody, clone 186-2G9 (Cat # MAB6936) at 10 mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration.
  • Application Image
  • Gene Information
  • Entrez GeneID:
  • 3385
  • Gene Name:
  • ICAM3
  • Gene Alias:
  • CD50,CDW50,ICAM-R
  • Gene Description:
  • intercellular adhesion molecule 3
  • Gene Summary:
  • The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. [provided by RefSeq
  • Other Designations:
  • intercellular adhesion molecule-3
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