MHC Class I monoclonal antibody, clone 3F10
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More Files
- More Functions
Flow Cytometry
5 X 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 uL of anti-MHC Class I (FITC) at a 1 : 50 dilution. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.77 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 uL of 0.5 mg/mL MHC Class I monoclonal antibody, clone 3F10 (Cat # MAB6933).
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Specification
Product Description
Mouse monoclonal antibody raised against native MHC Class I.
Immunogen
Native purified MHC Class I from human HSB-2 T cell line.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
5 X 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 uL of anti-MHC Class I (FITC) at a 1 : 50 dilution. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.77 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 uL of 0.5 mg/mL MHC Class I monoclonal antibody, clone 3F10 (Cat # MAB6933). -
Publication Reference
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A new foreign policy: MHC class I molecules monitor the outside world.
Rock KL.
Immunology Today 1996 Mar; 17(3):131.
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The essence of epitopes.
Barber LD, Parham P.
The Journal of Experimental Medicine 1994 Oct; 180(4):1191.
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Structure, function, and diversity of class I major histocompatibility complex molecules.
Bjorkman PJ, Parham P.
Annual Review of Biochemistry 1990 Jan; 59:253.
Application：WB-Ce, Human, Human B cells, Human T cells.
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A new foreign policy: MHC class I molecules monitor the outside world.
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