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Last updated: 2017/7/23
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MHC Class I monoclonal antibody, clone 3F10

  • Catalog # : MAB6933
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against native MHC Class I.
  • Immunogen:
  • Native purified MHC Class I from human HSB-2 T cell line.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
  • Storage Instruction:
  • Store at 4°C.
  • Publication Reference
  • Applications
  • Flow Cytometry
  • Flow Cytometry
  • 5 X 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 uL of anti-MHC Class I (FITC) at a 1 : 50 dilution. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.77 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 uL of 0.5 mg/mL MHC Class I monoclonal antibody, clone 3F10 (Cat # MAB6933).
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