GYPA monoclonal antibody, clone A63-B/C2
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More Files
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Flow Cytometry
107 human red blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD235a (Glycophorin A) /Biotin at 20 ug/mL. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.27 log10 fluorescent units when compared to a Mouse IgM/Biotin negative control at a similar concentration. Binding was 82% blocked when pre incubated 10 minutes with 20 uL of 0.5 mg/mL GYPA monoclonal antibody, clone A63-B/C2 (Cat # MAB6932).
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Specification
Product Description
Mouse monoclonal antibody raised against native GYPA.
Immunogen
Native purified GYPA from neuraminidase treated human erythrocytes.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgM
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 50 mM sodium phosphate buffer, 100 mM potassium Chloride, 150 mM NaCl, pH 7.5 (0.5 mg/mL gentamicin sulfate)
Storage Instruction
Store at 4°C.
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Applications
Flow Cytometry
107 human red blood cells were washed and preincubated 5 minutes with 20 uL of 250 ug/mL human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of anti-CD235a (Glycophorin A) /Biotin at 20 ug/mL. Cells were washed twice and incubated with Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.27 log10 fluorescent units when compared to a Mouse IgM/Biotin negative control at a similar concentration. Binding was 82% blocked when pre incubated 10 minutes with 20 uL of 0.5 mg/mL GYPA monoclonal antibody, clone A63-B/C2 (Cat # MAB6932). -
Gene Info — GYPA
Entrez GeneID
2993Gene Name
GYPA
Gene Alias
CD235a, GPA, GPErik, GPSAT, GpMiIII, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS
Gene Description
glycophorin A (MNS blood group)
Gene Ontology
HyperlinkGene Summary
Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq
Other Designations
Mi.V glycoprotein (24 AA)|erythroid-lineage-specific membrane sialoglycoprotein|glycophorin A|glycophorin A (MN blood group)|glycophorin A MNS blood group|glycophorin A, GPA|glycophorin Erik|glycophorin MiI|glycophorin MiIII|glycophorin MiV|glycophorin Mi
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Interactome
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Pathway
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Disease
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Publication Reference
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The expression of human blood group antigens during erythropoiesis in a cell culture system.
Southcott MJ, Tanner MJ, Anstee DJ.
Blood 1999 Jun; 93(12):4425.
Application：Flow Cyt, Human, Human placental cord blood cells.
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High-efficiency gene transfer into ex vivo expanded human hematopoietic progenitors and precursor cells by adenovirus vectors.
Frey BM, Hackett NR, Bergelson JM, Finberg R, Crystal RG, Moore MA, Rafii S.
Blood 1998 Apr; 91(8):2781.
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Characterization of a bipotent erythro-megakaryocytic progenitor in human bone marrow.
N Debili, L Coulombel, L Croisille, A Katz, J Guichard, J Breton-Gorius, W Vainchenker.
Blood 1996 Aug; 88(4):1284.
Application：Flow Cyt, Human, Human bone marrow cells.
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The expression of human blood group antigens during erythropoiesis in a cell culture system.
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