5 X 105 cultured human Raji cells were washed and incubated 45 minutes on ice with 80 ml of CD80 monoclonal antibody, clone P1.H5.A1.A1 (Cat # MAB6905) at 20mg/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
The B-lymphocyte activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.[supplied by OMIM