5 X 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 uL of CD19 monoclonal antibody, clone BU12 (Cat # MAB6901) at 10 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq