Glo1 monoclonal antibody, clone 6F10
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of Glo1 protein by Western blotting with Glo1 monoclonal antibody, clone 6F10 (Cat # MAB6768).
Samples are whole cell extracts :
Lane1 : GCOS-1 (simian).
Lane2 : GL929 (mouse).
Lane3 : GHeLa (human).
Mouse Glo1 shows a single band of 27 kDa while human and simian ones show 29 kDa.
Immunofluorescence
Immunofluorescent staining of HeLa cells with Glo1 monoclonal antibody, clone 6F10 (Cat # MAB6768).
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Specification
Product Description
Rat monoclonal antibody raised against full length recombinant Glo1.
Immunogen
Recombinant GST fusion protein corresponding to full length mouse Glo1.
Host
Rat
Reactivity
Human, Monkey, Mouse
Specificity
This antibody is specific to Glo1.
Form
Liquid
Isotype
IgG2b, kappa
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Detection of Glo1 protein by Western blotting with Glo1 monoclonal antibody, clone 6F10 (Cat # MAB6768).
Samples are whole cell extracts :
Lane1 : GCOS-1 (simian).
Lane2 : GL929 (mouse).
Lane3 : GHeLa (human).
Mouse Glo1 shows a single band of 27 kDa while human and simian ones show 29 kDa.Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of HeLa cells with Glo1 monoclonal antibody, clone 6F10 (Cat # MAB6768).Enzyme-linked Immunoabsorbent Assay
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Gene Info — Glo1
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Publication Reference
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Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice.
Hovatta I, Tennant RS, Helton R, Marr RA, Singer O, Redwine JM, Ellison JA, Schadt EE, Verma IM, Lockhart DJ, Barlow C.
Nature 2005 Dec; 438(7068):662.
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Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor.
Junaid MA, Kowal D, Barua M, Pullarkat PS, Sklower Brooks S, Pullarkat RK.
American Journal of Medical Genetics. Part A 2004 Nov; 131(1):11.
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Role for glyoxalase I in Alzheimer's disease.
Chen F, Wollmer MA, Hoerndli F, Munch G, Kuhla B, Rogaev EI, Tsolaki M, Papassotiropoulos A, Gotz J.
PNAS 2004 May; 101(20):7687.
Application：IHC, WB, Human, Mouse, Human brain, Mouse brain.
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Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice.
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