5-Methylcytosine monoclonal antibody, clone 5MC-CD
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More Files
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Immunofluorescence
Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766). Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs.
Immunofluorescence
Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamydomonas me-1 cells.
Left : DAPI-stained cells.
Middle : Cells stained with 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766) and FITC-conjugated 2nd antibody.
Right : Merged image Chloroplast DNA is exclusively methylated in gamete cells.
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Specification
Product Description
Mouse monoclonal antibody raised against 5-Methylcytosine.
Immunogen
5-Methylcytosine conjugated with BSA.
Host
Mouse
Form
Liquid
Isotype
IgM
Recommend Usage
Immunocytochemistry (1:50-1:100)
Western Blot (~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunocytochemistry
Immunofluorescence
Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766). Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs.Immunofluorescence
Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamydomonas me-1 cells.
Left : DAPI-stained cells.
Middle : Cells stained with 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766) and FITC-conjugated 2nd antibody.
Right : Merged image Chloroplast DNA is exclusively methylated in gamete cells. -
Publication Reference
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The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA.
Sharif J, Muto M, Takebayashi S, Suetake I, Iwamatsu A, Endo TA, Shinga J, Mizutani-Koseki Y, Toyoda T, Okamura K, Tajima S, Mitsuya K, Okano M, Koseki H.
Nature 2007 Dec; 450(7171):908.
Application：IF, Human, Human embryonic stem cells .
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A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes.
Nishiyama R, Ito M, Yamaguchi Y, Koizumi N, Sano H.
PNAS 2002 Apr; 99(9):5925.
Application：IF, Chlamydomonas reinhardtii, me-1 cells.
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Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine.
Sano H, Imokawa M, Sager R.
Biochimica et Biophysica Acta 1988 Nov; 951(1):157.
Application：AFC, IA, Bovine, Human, Bovine nuclear DNA, Human lymphocytes DNA.
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The SRA protein Np95 mediates epigenetic inheritance by recruiting Dnmt1 to methylated DNA.
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