Methylation of chloroplast DNA visualized by immunochemistry. Samples are Chlamydomonas me-1 cells. Left : DAPI-stained cells. Middle : Cells stained with 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766) and FITC-conjugated 2nd antibody. Right : Merged image Chloroplast DNA is exclusively methylated in gamete cells.
Detection of DNA methylation in mouse embryonic stem cells by immunofluorescence staining with the 5-Methylcytosine monoclonal antibody, clone 5MC-CD (Cat # MAB6766). Intense 5-methylcytosine staining at pericentromeric regions was seen in the mitotic chromosome and interphase nuclei of ESCs.