RB1 (phospho T821) monoclonal antibody, clone 24A7
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More Files
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Western Blot
Specificity of the RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765) as demonstrated by western blotting.
Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (Lane 1), Rb (S795A, lane 2) or Rb (T821A, lane 3) were immunoprecipitated with anti-Myc antibody and the precipitates were analyzed by western blotting with RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765).
Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic phosphopeptide of RB1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T821 of human RB1.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to human RB1 phosphorylated at Thr821. It can detect endogenous levels of RB1 phosphorylated at Thr821 in most cell lines.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C. Do not freeze by storing below -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Specificity of the RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765) as demonstrated by western blotting.
Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (Lane 1), Rb (S795A, lane 2) or Rb (T821A, lane 3) were immunoprecipitated with anti-Myc antibody and the precipitates were analyzed by western blotting with RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765).
Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RB1
Entrez GeneID
5925Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Cancer cell cycles.
C J Sherr.
Science 1996 Dec; 274(5293:1672.
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A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-Abl tyrosine kinase in the cell cycle.
Welch PJ, Wang JY.
Cell 1993 Nov; 75(4):779.
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E2F: a link between the Rb tumor suppressor protein and viral oncoproteins.
Nevins JR.
Science 1992 Oct; 258(5081):424.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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Cancer cell cycles.
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