Specificity of the RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765) as demonstrated by western blotting. Crude extracts of human lung carcinoma cell line H1299 transfected with plasmid expressing Myc-tagged wild-type Rb (Lane 1), Rb (S795A, lane 2) or Rb (T821A, lane 3) were immunoprecipitated with anti-Myc antibody and the precipitates were analyzed by western blotting with RB1 (phospho T821) monoclonal antibody, clone 24A7 (Cat # MAB6765). Data provided by Dr. Y. Inoue at the JFCR Cancer Research Institute, Tokyo.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein