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Last updated: 2017/12/10
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E2F1 (phospho S364) monoclonal antibody, clone #2

  • Catalog # : MAB6761
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against synthetic phosphopeptide of E2F1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S364 of human E2F1.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Specificity:
  • This antibody reacts with human E2F1 phosphorylated at Ser364.
  • Form:
  • Liquid
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In antiserum (0.05% sodium azide)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot
  • Immunoprecipitation
  • Immunoprecipitation
  • MCF-7 cells were grown in the absence (Lane 1) or in the presence of etoposide at 10 uM for 16 hr (Lanes 2 and 3).
    Crude lysates were prepared and analyzed by western blotting (Lane 3) with E2F1 (phospho S364) monoclonal antibody, clone #2 (Cat # MAB6761) or immunoprecipitated by pantropic anti-E2F1 antibody followed by western blotting with the antibody #2.
  • ELISA
  • Application Image
  • Western Blot
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1869
  • Gene Name:
  • E2F1
  • Gene Alias:
  • E2F-1,RBAP1,RBBP3,RBP3
  • Gene Description:
  • E2F transcription factor 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000030661,retinoblastoma-associated protein 1
  • Interactome
  • Interactome
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